^^^Yes just changed tyres on one bike (Aksiums) from Gatorskins to Lithion 2s, and there was a considerable difference in the circumference. The circumference of Gatorskins on Shimano 501s was different to the same tyres on the Aksiums too. I measure the diameter as accurately as I can using a spirit level held against the top of the wheel (held vertical), and then multiply by 3.14159