Nice one, loafer... here's few off the top of my head...
First 'proper' hill is at about 20 miles. I think it's called Ritton Bank.
Watch the road surface in the dip at Forestburn Gate (21.5 miles)... it's very potholed, and easy to pick up a pinch puncture here... try not to go down it in a group, so as you can pick your line well.
Miles 23-24 = long haul up, looks worse than it is. Nice fast downhill after.
Miles 43-45 (Billsmoor) is what I'd call a 'significant' hill.
Another big hill out of Otterburn, from about miles 53-56, but I've never ridden this one.
Just after Bellingham, at about mile 68, there's a hill I'd call "a bit of a bu99er"
2 absolute sods just before Ryal (about mile 83)... straight road, two very steep climbs one after the other with little recovery time between them. They're the last big test, and it's pretty much easy going after that.
Generally road surfaces good, but in places tend to be potholed at edges, so keep away from the sides if you can.
The ride itself (I did 60 miler last year) was excellently organised, and timing stops have more bananas than a troupe of monkeys could ever want, plus lots of isotonic drinks, etc. Was a great atmosphere, and I had loads of good chats going round too. I only saw one accident (fella with an artificial leg who fell off and got hit by following bike, cutting his cheek... claret everywhere, there was).